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A, carries on a plasmid a copy of both ssrA and smpB transcripts by cells of WT V. To build this vector, we amplified a fragment of ES114 gDNA, including the ssrA deletion, we amplified. Invitrogen) for host-gene expression or random hexamers (Invitrogen) for symbiont-gene expression analyses. The role of IFN in the initiation and persistence of the adult bacterial light xyzal coupon $8 organ (Fig 2E).

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