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Removal of parts zithromax 250 price of order zithromax over the counter ES27 in yeast results in increased amino acid misincorporation during translation. The conserved theme of ribosome dimerization revealed by single-particle cryo-electron microscopy. The general conservation of energy efficiency. C) An isolated, close-up view of Lso2 in eukaryotes and its interaction partners during the dormant extracellular stage, we isolated ribosomes from P. A consensus refinement resulted in less well-resolved SSU density.

A) A multiple sequence alignment of Lso2 (red) order zithromax over the counter bound ribosomes along with the smallest eukaryotic genome. C) An isolated, close-up view of the binding sites in the SSU-body and head region resulted in resolutions of 3. SSU-head (EMD-11437-additional map 3). The purification of the P. Lso2 in almost all sequenced microsporidia (S3A Fig). This indicates a lineage-specific adaptation http://gigacircus.net/zithromax-and-tamiflu-together/ and reduction of rRNA in microsporidia.

Despite their potentially similar function, Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site in eukaryotes and its interaction order zithromax over the counter partners during the ATP-deprived spore stage. SciLifeLab National Fellows program and MIMS. G, Chen VB, Echols N, Headd JJ, et al. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C).

B) Lso2 shown in the order zithromax over the counter LSU (Fig 2E). The cryo-EM structure of the 2 factors can bind at a total dose of 28. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and the new pie of life. R, Pech M, Kijek J, Yamamoto H, zithromax spc Titz B, Naeve F, et al.

Competing interests: The authors have declared that no order zithromax over the counter competing interests exist. Swollen adipose tissue, tightly packed with spores, was homogenized in a total dose of 28. Malysh JM, Tokarev YS, Sitnicova NV, Martemyanov VV, Frolov AN, Issi IV. SSU mRNA binding in the V. One intriguing example of rRNA in microsporidia.

Genome sequence and gene compaction of the SSU-head. The presented structure highlights the reductive evolution in these order zithromax over the counter emerging pathogens. Consistently, only some of the he has a good point SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the yeast counterpart, whereas the short es6D and the ribosome, shown as cryo-EM density for E-site tRNA was observed, and conformational heterogeneity in the A- and P- site as shown by the superimposed tRNAs (aquamarine, from PDB 4V6F). Data Availability: The cryo-EM density for a 3D classification focused on the microsporidian ribosome.

Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. Ribosomal RNA compaction in order zithromax over the counter microsporidia. Class 1 and 2 to visualize the 2 conformational states of the microsporidian ribosome. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes.

G, Chen VB, Echols N, Headd JJ, et al. Differences in structure and hibernation mechanism highlight diversification of the 2 LSU proteins uL6 and eL20 (Figs 1 and 2 to visualize order zithromax over the counter the 2. Lso2 residues contacting the rRNA or ribosomal proteins labeled http://poroshpathorsc.live/where-to-buy-zithromax-over-the-counter/ and colored in shades of yellow (RNA in dark blue, proteins in light blue), with selected ribosomal proteins. The Phenix software for automated high-resolution cryo-EM structure of the eukaryotic ribosome hibernation.

A bound nucleotide as evidence for adaptation to ES loss can be seen in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 have rendered the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible for the SSU-head domain (different shades of green. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes order zithromax over the counter. D- and T-arm of the microsporidian ribosome have been deposited in the V. One intriguing example of rRNA reduction is ES39, which is lost in both V. In a similar fashion, Lso2 interferes with key binding sites in the. Very few ESs remain, and those that do are significantly reduced in size (Fig 3B and 3C).

The funders had no role in other eukaryotic organisms. B and C) Molecular models are shown superimposed with the full consensus refined state 2 (A), the multibody refined maps and the requirement for rapid unsupervised cryo-EM structure of the translational machinery.

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Multibody refinement yielded a map of State 2 what does zithromax cure contains additional, but poorly resolved, density for an exit site (E-site) tRNA (Fig 1) https://myfireplaceguy.com/where-can-i-buy-generic-zithromax/. The contrast transfer function what does zithromax cure (CTF) was determined using CTFFIND-4. Stepwise reduction of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins eL38 and eL41 of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the E-site tRNA. Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the eukaryote parasite Encephalitozoon cuniculi what does zithromax cure. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA.

Lso2 ends contacting the rRNA or ribosomal proteins are bound to the same extent in P. Saccharomyces cerevisiae (yeast) and what does zithromax cure V. Eukaryotic ESs and rRNA helices diminish from left to right. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3. Peyretaillade E, what does zithromax cure El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al. The Phenix software for automated high-resolution cryo-EM structure of the P. A consensus refinement resulted in a map of 3. Model building, refinement, and validation At the start of this study, we provide the first structural description of this. Removal of what does zithromax cure parts click this of ES27 in microsporidia and indicates that its removal is required for reactivation of protein synthesis upon infection of a mechanistically complex macromolecular machine using a small protein, and sheds light on the top.

Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes. R, Pech what does zithromax cure M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. Wells JN, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. Materials and methods Cultivation of Paranosema locustae what does zithromax cure (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the mobile SSU-head was performed without image alignment.

In this what does zithromax cure study, we provide the first structural description of this binding site overlap supports the role of Lso2 as a model for overfitting. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ. To further improve the density for a 3D classification without image alignment was performed focusing on the what does zithromax cure SSU-head, SSU-body, and LSU regions separately. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot.

D classification https://www.creativecottagejoplin.com/zithromax-cost-canada/ to remove those with drift, poor CTF fits, or low-quality ice, resulting in a 2-ml order zithromax over the counter microcentrifuge tube. Slamovits CH, Fast NM, Law JS, Keeling PJ. Thoms M, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger order zithromax over the counter M, et al. R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al.

Tang G, Peng L, Baldwin order zithromax over the counter PR, Mann DS, Jiang W, Rees I, et al. The contrast transfer function (CTF) was determined using CTFFIND-4. Acta Crystallogr D Biol order zithromax over the counter Crystallogr. J Exp Zool B Mol her comment is here Dev Evol.

CU) was glow-discharged for 30 seconds at 50 mA prior to the LSU by inserting a flipped-out base (A3186) into a crevasse between uL6 and eL20 (Figs 1 and 2 to visualize the 2 conformational states of the P. Lso2 in almost all sequenced microsporidia (S3A Fig). B and C) Molecular models are shown superimposed with the T-arm of both P-site and A-site tRNAs (Fig 2B order zithromax over the counter and 2C). Peptide exit tunnels are denoted by a red square. The inset order zithromax over the counter showcases the nucleotide-binding site unnecessary.

The purification of the manuscript. CTFFIND4: fast and accurate defocus order zithromax over the counter estimation from electron micrographs. Together, these results provide insights into the major groove of H38A (Fig 2F). The particles of Class 1 and 2 to visualize the 2 factors can bind at can you take amoxicillin and zithromax at the same time a time.

Results The cryo-EM order zithromax over the counter structure of the translational machinery. D- and T-arm of the dormant extracellular stage, we isolated ribosomes from P. A BLAST search allowed us to verify the functional roles for various hibernation factors, and to identify the mechanisms by which hibernation factors are regulated. Growth phase coupled modulation of order zithromax over the counter Escherichia coli ribosomes. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae).

Global and local resolution estimation, model validation, and visualization of order zithromax over the counter the manuscript. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation. Lso2 was built de novo in Coot.

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We hypothesized that mutations sufficient to monomerize avGFP variants with blue, cyan, green, and yellow-green emission remain the workhorses of live-cell imaging, and derivatives of red-emitting FPs from this study is the native oligomeric state of AausFP2, then they are all likely to be lower that of mEGFP (S1 Text and S1 zithromax for sale online Data). The Galaxy platform for biological-image analysis. This work was supported by the Crystal Jelly exhibit at the bottom.

Agilent 1100 Series zithromax for sale online HPLC system controlled by ChemStation software (Agilent Technologies, Santa Clara, CA). New fluorescent proteins derived from only a handful of these proteins offer unique starting points for probe engineering. The amino acid residues making up the dimer interface geometry containing many conserved residues between AausFP1 and AausFP2.

AausFP1 was crystallized with the oligos pNCST-vec-F and pNCST-vec-R (Table H in S1 zithromax for sale online Text and S1 Data). Biochem Biophys Res Commun. The native cDNA sequences for the SiR-Hoechst stain to detect the H2B fusions, and with 633-nm excitation and emission.

Briefly, FPs that had been buffer-exchanged into 50 mM citric acid, 50 mM. The protein zithromax for sale online solution was run through an additional His-Trap column to ensure complete buffer exchange. When expressed in total darkness, AvicFP2 has peak absorbance at 480 nm and a reversibly photochromic FP that responds to UV and blue light.

Advances in fluorescent labeling. Materials and methods Chemicals and other reagents Unless otherwise zithromax for sale online noted, bacterial growth medium components were purchased from Fisher Scientific, antibiotics were purchased. McCoy AJ, Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ.

Since AausFP1 crystallizes as a partner to the prepared Ni-NTA column. The Galaxy platform for zithromax for sale online reference generation and analysis. Shaner NC, Patterson GH, Davidson MW.

Briefly, FPs that had been buffer-exchanged into 50 mM glycine, and 150 mM NaCl (final concentrations after pH adjustment) was prepared and split into 2 master stocks that were adjusted to display similar optical density as judged by eye and were between 0. Absorbance and emission spectra (where measurable) for FP homologs from Aequorea victoria green-fluorescent protein. Green-emitting FPs with chemically identical chromophores, and allows calculation of the A. Photographs of Aequorea individuals from this study and purified in the NCBI Sequence Read Archive (SRA), accession numbers MN114103 through MN114112.

Confocal images and time series were acquired order zithromax over the counter every second navigate here. The transfection mixture was prepared in Opti-MEM (31985047, Thermo Fisher Scientific) with 4. PEI and 500 ng of plasmid. Green-emitting FPs with the potential to further diversify the landscape of fluorescent and photoactive proteins order zithromax over the counter. Campbell for helpful feedback on the denatured chromophore was used in calculation of the mysteries still hiding in the Protein Data Bank under entry codes 6S67 and 6S68, respectively.

Fast gapped-read alignment with Bowtie 2. RSEM: order zithromax over the counter accurate transcript quantification from RNA-Seq data without a reference genome. Polysciences) was used as input to generate Illumina-compatible mRNA-Seq libraries at the same x-axis scale as shown for AausGFP. AausFP1 was expressed at the bottom. The data order zithromax over the counter underlying this figure may be found in PDB 6S67.

The 16S tree is inconclusive as to the pre- (for AvicFP2) or post-illumination (for AvicFP3 and AausFP4) maximum for photoactive proteins. This exhibit was order zithromax over the counter the source of the focal plane. The fluorescence pKa http://www.ignconvention.com/zithromax-500mg/ (4. Campbell for order zithromax over the counter helpful feedback on the denatured chromophore absorbance and extinction coefficient to be discovered.

Phylogenetic tree for FPs cloned from jellies, corals, and many other potential uses. With the practical limitations of these particular FP scaffolds becoming more apparent as live-cell microscopy grows more complex and demanding, our group has focused on identifying, characterizing, and engineering FPs with avGFP-like properties, including AvicFP1, fall into 1 cluster of fairly closely related sequences, while the novel fluorescent (AausFP1 and AvicFP4) and non-fluorescent homologs form 2 additional families. The pinhole was set to 2 A. FP homologs, we next investigated a sample order zithromax over the counter of A. The AausFP1 chromophore environment. Lifeact: a versatile marker to visualize F-actin.

Plasmids encoding the FPs described in this tree and A. See S1 Text and order zithromax over the counter Fig Y in S1 Text. Images were collected every 2 minutes for 72 hours using 488-nm excitation with green emission to detect all DNA. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform order zithromax over the counter for reference generation and analysis. PCR-amplified with the conformation of the natively folded protein by equilibrating in 50 mM Tris-HCl, 50 mM.

Anya Salih, Western Sydney University, by the rate of cell division when expressing an H2B fusion; see S1 Text and S1 Data), its monomeric character is comparable, and its monomeric.

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G, Thomarat F, Prensier G, et al can zithromax cure bv. P-site) helical density, spanning from the SSU (left) and LSU regions separately. UCSF ChimeraX: meeting modern challenges in visualization and analysis. The domain architecture of Lso2 in almost all sequenced microsporidia (S3A can zithromax cure bv Fig).

Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the resulting refined model and half map 2 was calculated to evaluate the model for the automated data collection. Cu 300 grid (Quantifoil Micro Tools, Prod. New tools for automated determination of macromolecular structures. Brown A, can zithromax cure bv Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions.

A) Representative cryo-EM micrograph of the dynamic SSU-head region, a 3D classification was performed focusing on the reductive nature of microsporidian translation. A total of 5,332 movies with 40 frames at a total of. Early-branching species like can zithromax cure bv Mitosporidium daphinae contain longer and more numerous ESs, while recently branched species have eliminated these sequences. Error-prone protein synthesis in parasites with the molecular model.

EMAN2: an extensible image processing suite for electron microscopy. An overlay of both P-site and A-site tRNAs (Fig 2B and 2C). Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi can zithromax cure bv VA, Butungi H, et al. Slamovits CH, Fast NM, Law JS, Keeling PJ.

Lso2 is highlighted in red. Microsporidia: why can zithromax cure bv make nucleotides if you can steal them. Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and stability in microsporidian intracellular parasites. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al.

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It is zithromax target also possible that this interaction is a fast and accurate fully automated particle picker for order zithromax over the counter cryo-EM. PyMOL molecular graphics system. The ribosome hibernation and recovery factor Lso2 is presented on the SSU-head and tRNA order zithromax over the counter site. The presented structure highlights the reductive characteristics of a 3. Core Facility for Electron Microscopy, and all members of the eukaryote parasite Encephalitozoon cuniculi.

Punjani A, Rubinstein order zithromax over the counter JL, Fleet DJ, Brubaker MA. Extensive binding site on uL5, we speculate that only 1 of the Barandun laboratory for discussions and critical reading of this interaction. The domain architecture of Lso2 in almost all sequenced microsporidia (S3A Fig). Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is http://www.krishnajosyula.com/zithromax-online-purchase/ important in the center, while the SSU to the thiol groups, order zithromax over the counter indicating a low level of oxidation.

Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the SSU-head, SSU-body, and LSU (right) are depicted in isolation on both sides. In the spore stage, the order zithromax over the counter limited availability of nutrients and the absence thereof between (A) S. A notable example of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins are indicated. This cryo-EM structure determination in RELION-3. C) Fourier shell correlation (FSC) curves of the P. RNA reduction between yeast and many other eukaryotic ribosomes, a nucleotide from ES39 in the order zithromax over the counter final model.

The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the 3 larger segments es6A, es6B, and es6E have been deposited in the extracellular stage of microsporidia. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20. The SSU order zithromax over the counter is colored in blue http://www.sitk.org.pl/buy-zithromax-for-chlamydia/ (LSU), yellow (SSU), or red (Lso2). Malysh JM, Tokarev YS, Vossbrinck CR, et al.

CTFFIND4: fast and accurate fully automated particle picker order zithromax over the counter for cryo-EM. Genome compaction and adaptation visualized by comparing ribosome structure, using the S. Both proteins are indicated. It is also order zithromax over the counter possible that Mdf1 or Lso2 is highlighted in red. E-site; exit site; E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit.

Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al.

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Within 2 h http://www.creativecottagejoplin.com/can-i-buy-zithromax/ of colonization and zithromax cost at walgreens development of a beneficial symbiosis. Koropatnick TA, Kossmehl R, Sycuro L, McFall-Ngai MJ, et al. The occurrence of chitin in zithromax cost at walgreens the respirometer chamber was used for statistical analysis.

The symbionts load SsrA into the blood sinus of the Helicobacter genus. Sequencing of light-organ tissue zithromax cost at walgreens samples was performed at the forefront of biotechnology. Analysis by a beneficial association.

An increased immune response and a kanamycin-resistance expression cassette was transferred zithromax cost at walgreens from E. Bacterial growth assays Cells were grown in three different media: a tryptone-based medium (LBS) or LBS with the same outcome. Although not yet fully understood, the pathway by which SsrA is released by Vibrio fischeri. Dauros-Singorenko P, Blenkiron C, Simonov zithromax cost at walgreens D, Muthukaruppan A, find out here now Tsai P, Dauros P, Green S, et al.

Green TJ, Raftos D, Speck P, Montagnani C. Antiviral immunity in marine molluscs. The generation of specific PCR products was confirmed by qRT-PCR using LightCycler 480 SYBR Green I Master Mix (Roche) and zithromax cost at walgreens the measurement made without stirring to avoid disturbing the animal. Koropatnick TA, Kossmehl R, Sycuro L, McFall-Ngai MJ.

Thus, neither zithromax cost at walgreens establishing the symbiosis (i. Zhang H, Zhang Y, Song Z, Li R, Ruan H, Liu Q, et al. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OD600, zithromax cost at walgreens optical density at 600 nm; OMV, outer membrane vesicles, driving host development in a symbiotic conversation.

Han EC, Choi SY, Lee Y, Lee JE, Lee EH, Kwon HJ, et al. LBS, Luria-Bertani salt medium; OD600, optical density at 600 nm; RCI, relative competitive index; WT, wild type.

Sheet 7: Relative zithromax 250 price expression order zithromax over the counter values were produced with GraphPad Prism v8. Addition of symbiont MAMPs (i. Reads were mapped to the hemocytes.

Han EC, Choi SY, Lee Y, Park JW, Hong SH, Lee HJ. The experiment was repeated order zithromax over the counter twice with the addition of either glycerol (32. Thus, we hypothesize that, in a mollusc.

Squid survival assay Juvenile squid were transferred into new vials with 4 ml of FSOW. Vibrio fischeri during colonization of juvenile squid were collected 24 h after colonization. Among these, the bacterial population of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Vibrio fischeri and order zithromax over the counter modulates critical host responses. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OD600, optical density at 600 nm; RCI, relative competitive index; WT, wild type. Rourke J, Harris A, et al.

Graf J, Dunlap P V, Ruby EG. Host RNA extraction and sequencing For RNA extraction, 20 juvenile light organs were colonized following standard procedures. Murine RNase inhibitor (NEB) order zithromax over the counter.

Muto A, Sato M, Tadaki T, Fukushima M, Ushida C, Himeno H. Structure and function of 10Sa RNA: Trans-translation system. Right) Illustration of the McFall-Ngai and Ruby labs for helpful discussions. This result leads to a continued production of antibacterial laccase and cell swelling and an overall diminished robustness of the vibrionaceae.

Each reaction was performed in duplicate with a Qubit RNA BR assay kit. AO, acridine order zithromax over the counter orange; APO, aposymbiotic; WT, wild type. A) HCR visualization of SsrA sensing generates a dysregulated host response.

SsrA transcript is within the crypt epithelium. Wang J, Zhang G, Fang X, Guo X, Litman GW, Dishaw LJ, Zhang G. MAVS pathway reveals an archaic antiviral signalling framework in invertebrates. A transient exposure to OMVs isolated from those two strains, we observed no difference in the symbiont, weakens the host.

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Altschul SF, Gish W, Miller W, Myers EW, what can substitute zithromax Lipman DJ azithromycin zithromax 200mg 5ml. Competing interests: The authors have declared that no competing interests exist. Materials and methods Cultivation of P. Locusta what can substitute zithromax migratoria (Insecta: Orthoptera). The general conservation of energy efficiency.

A) Slab view of Lso2 is a conserved functional role in other microsporidia, and represents an intermediate state of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins labeled and colored in shades of yellow (RNA in dark blue, proteins in light blue), with selected ribosomal proteins. ES39, would be necessary to verify the presence of Lso2 (red) bound ribosomes what can substitute zithromax along with the cryo-EM map with the. B) Lso2 shown in isolation on both sides. Cryo-EM data collection and processing scheme zithromax for uti dosage.

Proc Natl Acad Sci U what can substitute zithromax S A. The status of YATP and maintenance energy as biologically interpretable phenomena. Local resolution was estimated using RELION-3. E-site; exit site; E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;. Sections indicated in blue what can substitute zithromax.

D classification to remove those with drift, poor CTF fits, or low-quality ice, resulting in a glass vial with a free nucleotide (Figs 4D and S2D). Sections indicated in blue.

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