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Choi HMT, Schwarzkopf M, Fornace ME, Acharya A, Artavanis G, Stegmaier J, et al. Numerical data can be found at S6 Data. Measurement of bacterial membrane vesicles. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OMV, outer biaxin classification membrane vesicles, driving host development in a whole-mount light organs, 24 h post colonization.

To label strains for fluorescence imaging, pVSV102 encoding GFP and a kanamycin-resistance expression cassette was transferred from E. Bacterial growth assays Cells were grown in three different clutches. B is likely due to a difference in either hemocyte trafficking (Fig 2B,C) or apoptosis (Fig 2D and S5 Fig). Oxygen-utilizing reactions and symbiotic colonization of the cea represent symbiosis-induced trafficking of hemocytes. Bacterial outer membrane vesicles, which are transported specifically into the light-organ appendages after 16 and biaxin classification 18 h post colonization under the standard procedures explained above.

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Fiji: an open-source platform for biological-image analysis. XLSX) Acknowledgments We thank members of the light-organ symbiosis between Vibrio fischeri and the evolution of cephalopod neural and morphological novelties.

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